LOCUS       sequence-146199-        3846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Dlx3. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Dlx3
FEATURES             Location/Qualifiers
     source          1..3846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(971..989)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(972..989)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1007..1023)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1007..1023)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1020..1042)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1031..1047
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1055..1061,1062..1079,1080..1085))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1100..1121
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1238..1255)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1409..1997)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1489..1508)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2168..2959,2960..3028))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2791..2810
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3029..3133)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3211..3666)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3348..3369)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3560..3579
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3792..3814
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3806..3823
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3807..3823
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3830..3846,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3830..3846,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tcatcttccc attccacgcc agcccctgcc cgcaatccgc tgcccccacc gctcccatac
      121 agtgcctccc ccaactacct ggacgacccc accaactcct ggtaccacac acagaacctc
      181 agtggacccc acttacagca gcagcctcct cagccggcta ccctgcacca tgcctcccct
      241 gggcccccgc ctaaccctgg ggctgtgtac tgagtaccca cctggcctgc gcccctccac
      301 gaaggacccc ctccaggacc aggcagaagg tgccctgtcc tagcgacact caggaatcat
      361 tgaggggcac agggggaaag actcccttcc ctctcccttg tcccttcttc cagggaccca
      421 acaacctcca gatgacaaat gcatggaccg aggatgcccc ccaatctccc tccccttgct
      481 tagactgggg tgccctccag acggcgagga gttctacccc agtggggaca gcacatgctc
      541 tctgctccag gaacccggat tgcctctaga tggctcatcg ctttccagct tttcaaacac
      601 agtagagacc tccaaaatgg gagccagagt gtttgcaggt ccacctgtgc tggggcacca
      661 ggcgccacgg attccagcac agccagacct aaagcaccaa ggggcaacta tacattcttg
      721 agccactctt taaaagacat tggacattac atcatggctg atcctcaaga tggggggggg
      781 ctgacctagt ccccactgtt ctccagccct cattaggatt tgagggtcca aaccaaagaa
      841 aactccccca aatgagggaa cctttgatat ccagggcttc caagggaaag aatcactagt
      901 gaattcgcgg ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata
      961 gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc
     1021 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1081 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1141 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1201 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1261 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1321 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1381 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1441 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1501 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1561 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1621 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1681 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1741 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1801 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1861 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1921 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1981 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2041 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2101 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2161 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2221 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2281 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2341 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2401 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2461 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2521 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2581 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2641 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2701 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2761 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2821 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2881 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2941 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3001 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3061 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3121 taggggttcc gcgcacattt ccccgaaaag tgccacctga tgcggtgtga aataccgcac
     3181 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat
     3241 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3301 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3361 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3421 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3481 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3541 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3601 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3661 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     3721 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     3781 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
     3841 actata
//
