LOCUS       sequence-146206-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of LC3 fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Lee IH, Cao L, Mostoslavsky R, Lombard DB, Liu J, Bruns NE, Tsokos 
            M, Alt FW, Finkel T
  TITLE     A role for the NAD-dependent deacetylase Sirt1 in the regulation of 
            autophagy.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Mar 4. 105(9):3374-9.
  PUBMED    18296641
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Mar 4. 105(9):3374-9."
COMMENT     Sequence Label: pEGFP-LC3 (human)
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(135..509)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     primer_bind     complement(597..618)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga
      121 tccggtggat cctgcactga caatttcatc ccgaacgtct cctgggaggc atagaccatg
      181 tacaggaatc catcttcatc tttctcactc tcatacacct ctgagattgg tgtggagacg
      241 ctgaccatgc tgtgtccgtt caccaacagg aagaaggcct gattagcatt gagctgtaag
      301 cgccttctaa ttatcttgat gagctcactc atgttgacat ggtcaggtac aaggaacttt
      361 gttttatcca gaacaggaag ctgcttctca cccttgtatc gttctattat caccgggatt
      421 ttggttggat gctgctctcg aataagtcgg acatcttcta ctctttgttc gaaggtgcgg
      481 cgctgcttga aggtcttctc cgacggcatg gtcgggaatt cgaagcttga gctcgagatc
      541 tgagtccggc cggacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      601 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      661 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      721 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
      781 accttgatgc cgttcttctg cttgtc
//
