LOCUS       sequence-146395-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Vardy E, Robinson JE, Li C, Olsen RH, DiBerto JF, Giguere PM, 
            Sassano FM, Huang XP, Zhu H, Urban DJ, White KL, Rittiner JE, 
            Crowley NA, Pleil KE, Mazzone CM, Mosier PD, Song J, Kash TL, 
            Malanga CJ, Krashes MJ, Roth BL
  TITLE     A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation 
            of Behavior.
  JOURNAL   Neuron. 2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 
            10.1016/j.neuron.2015.03.065.
  PUBMED    25937170
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 
            10.1016/j.neuron.2015.03.065."
COMMENT     Sequence Label: pAAV-hSyn-dF-HA-KORD-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..230
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      272..281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    282..744
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(442..461)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     671..688
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
       61 aagcacatca ggccgtagcc gaaggtggtc acgagggtgg gccagggcac gggcagcttg
      121 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      181 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      241 accccggtga acagctcctc gcccttgctc accatggtgg cttatcatcg tgtttttcaa
      301 aggaaaacca cgtccccgtg gttcgggggg cctagacgtt tttttaacct cgactaaaca
      361 catgtaaagc atgtgcaccg aggccccaga tcagatccca tacaatgggg taccttctgg
      421 gcatccttca gccccttgtt gaatacgctt gaggagagcc atttgactct ttccacaact
      481 atccaactca caacgtggca ctggggttgt gccgcctttg caggtgtatc ttatacacgt
      541 ggcttttggc cgcagaggca cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc
      601 gctacagacg ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt cacgacattc
      661 aacagacctt gcattccttt ggcgagaggg gaaagacccc taggaatgct cgtcaagaag
      721 acagggccag gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac
      781 atatagacaa acgcacaccg gccttattcc aagcggcttc ggccagtacg ta
//
