LOCUS       sequence-146411-        3885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Pou2f1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Pou2f1
FEATURES             Location/Qualifiers
     source          1..3885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1010..1028)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1011..1028)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1046..1062)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1046..1062)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1059..1081)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1070..1086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1094..1100,1101..1118,1119..1124))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1139..1160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1277..1294)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1448..2036)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1528..1547)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2207..2998,2999..3067))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2830..2849
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3068..3172)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3250..3705)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3387..3408)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3599..3618
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3831..3853
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3845..3862
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3846..3862
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3869..3885,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3869..3885,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tctatggaga gtgaagatgc cagcacaggc acacaaacca atggtctgga ctttcagaaa
      121 cagcccgtgc ccgttggagg agcgatctcc acagcccagg cccaggcctt cctcggacat
      181 cttcaccagg tccagctagc tgggacaagt ttacaggctg ctgctcagtc tttaaatgta
      241 cagtctaaat ccagtgaaga gtcgggagat tcgcagcagt cgagccagcc ttcttcccag
      301 ccgccttcag tgcagtcagc cattccccag acccagctaa tgctggctgg gggacagata
      361 actgggctca cgttgacccc agcccagcaa cagttactgc tacagcaggc gcaggcccag
      421 gcccagctcc tggccgctgc agtgcagcaa cactccgcca gccaacagca cagtgctgct
      481 ggggccacca tctcagcctc cgccgccaca cccatgacgc agatccccct gtctcagccc
      541 atacagattg cacaggatct tcaacaattg caacagcttc agcagcaaaa tctcaacttg
      601 caacagtttg tgttggtgca cccaaccacc aacctgcaac cagcacagtt tatcatctca
      661 cagacccccc agggccagca gggtctcctg caagcgcaaa atcttttaac gcaactacct
      721 cagcaaagcc aagccaacct cctacagcca cagccaagca tcaccctcac gtcccagcct
      781 accaccccaa ctcgcacaat agcagcagcc tcagttcaga cacttccaca gagccagtca
      841 acaccaaagc gaattgacac tcccagcttg gaggagccca gtgaccttga ggagcttgag
      901 cagtttgcca agactttcaa acaaagacga atcactagtg aattcgcggc cgcctgcagg
      961 tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc
     1021 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1081 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1141 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1201 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1261 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1321 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1381 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1441 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1501 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1561 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1621 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1681 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1741 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1801 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1861 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1921 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1981 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2041 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2101 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2161 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2221 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2281 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2341 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2401 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2461 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2521 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2581 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2641 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2701 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2761 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2821 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2881 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2941 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3001 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3061 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3121 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3181 cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     3241 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     3301 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     3361 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     3421 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     3481 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     3541 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     3601 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     3661 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     3721 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     3781 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     3841 acgttgtaaa acgacggcca gtgaattgta atacgactca ctata
//
