LOCUS       sequence-146424-        3580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Tshz2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Tshz2
FEATURES             Location/Qualifiers
     source          1..3580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(705..723)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(706..723)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(741..757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(741..757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(754..776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    765..781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(789..795,796..813,814..819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    834..855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(972..989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1143..1731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1223..1242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1902..2693,2694..2762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2525..2544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2763..2867)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2945..3400)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3082..3103)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3294..3313
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3526..3548
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3540..3557
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3541..3557
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3564..3580,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3564..3580,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tcagcagaac ttgccttccc ggtctgtctc taagcccagc ctattcagct ccgtgcagct
      121 ataccggcag agcagtaagt tgtgtggctc ggtgttcacc ggcgccagca ggttccgctg
      181 caggcagtgc agtgcggcct acgacactct cgtggagctg accgtgcaca tgaacgagac
      241 aggccactat caagatgaca accgcaaaaa agataagctc agacccacga gctactccaa
      301 gccccgaaaa agggccttcc aagacatgga caaagaggat gcccaaaagg tcctgaaatg
      361 tatgttttgt ggtgactcct tcgattccct gcaagatttg agtgtccaca tgataaagac
      421 aaaacattac caaaaagtgc ctttgaagga gccggtacca accatttcct ccaagatggt
      481 cacaccagcc aagaagcgtg ttttcgatgt caacaggcct tgctcccctg actccaccac
      541 agggtccctt gcagattcct tttcttccca gaagagtgct aacctgcagc tgccctccaa
      601 cagccgctac ggctaccaaa acggaatcac tagtgaattc gcggccgcct gcaggtcgac
      661 catatgggag agctcccaac gcgttggatg catagcttga gtattctata gtgtcaccta
      721 aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      781 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      841 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      901 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      961 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2641 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2701 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2881 aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     2941 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     3001 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3061 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3121 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3181 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3241 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3301 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3361 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc
     3421 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     3481 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     3541 gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//
