LOCUS       sequence-146570-         465 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the 
            nucleotide-binding domain) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-Flag-NLRP3 91-710
FEATURES             Location/Qualifiers
     source          1..465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atagggagac ccaagcttgg taccgagctc ggatcgccca ccatggacta caaagacgat
       61 gacgataaaa tcgatatcga attcgagtgg aatgacacgt gtacatcaca ttcctctatg
      121 gtatgccagg aggacagcct tgaagaagag tggatgggtt tgctgggata tctctcccgc
      181 atctccattt gtaaaaagaa gaaagattac tgtaagatgt acagacgaca tgtgagaagc
      241 aggttctact ctatcaagga caggaacgcg cgtctaggtg agagtgtgga cctcaacagt
      301 cgctacacgc agctccaact ggtcaaggag catccaagca agcaggagcg ggagcatgaa
      361 ctcctgacca tcggccggac taaaatgcgg gacagcccca tgagttccct taagctggag
      421 ctgctgtttg agcccgagga cgggcactcg gagcctgtgc acaca
//
