LOCUS       sequence-146572-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-Flag-Caspase-1
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcgcccac catggactac aaagacgatg acgataaaat
      121 cgatatcgaa ttcatggctg acaagatcct gagggcaaag aggaagcaat ttatcaactc
      181 agtgagtata gggacaataa atggattgtt ggatgaactt ttagagaaga gagtgctgaa
      241 tcaggaagaa atggataaaa taaaacttgc aaacattact gctatggaca aggcacggga
      301 cctatgtgat catgtctcta aaaaagggcc ccaggcaagc caaatcttta tcacttacat
      361 ttgtaatgaa gactgctacc tggcaggaat tctggagctt caatcagctc catcagctga
      421 aacatttgtt gctacagaag attctaaagg aggacatcct tcatcctcag aaacaaagga
      481 agaacagaac aaagaagatg gcacatttcc aggactgact gggaccctca agttttgccc
      541 tttagaaaaa gcccagaagt tatggaaaga aaatccttca gagatttatc caataatgaa
      601 tacaaccact cgtacacgtc ttgccctcat tatctgcaac acagagtttc aacatctttc
      661 tccgagggtt ggagctcaag ttgacctcag agaaatgaag ttgctgctgg aggatctggg
      721 gtataccgtg aaagtgaaag aaaatctcac agctctggag atggtgaaag aggtgaaaga
      781 atttgctgcc tgcccagagc acaagacttc tgacagtact ttccttgtat tcatgtctca
      841 tggtatccag gagggaatat gtgggaccac atactcta
//
