LOCUS       sequence-146577-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; 
            kinase dead) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-HA-NEK7 K64M
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             360..368
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttacc atgggatacc cctacgacgt ccccgactac gccggatcca tggatgaaca
      121 atcacaagga atgcaagggc cgcccgttac tcagttccag ccacagaagg cattacggcc
      181 agatatgggc tataatactt tagccaactt ccgaatagaa aagaaaattg gtcgtggaca
      241 atttagtgaa gtttatagag catcctgtct cttggatgga gtgccggtag cgttaaaaat
      301 ggtacagata tttgatttaa tggatgccaa agcacgtgct gattgtatca aagaaataga
      361 cctccttaag caactcaacc atccaaatgt aattaaatac tatgcatcat tcattgagga
      421 taatgagctg aacatagttt tggagttagc agatgctggt gacctctcca gaatgataaa
      481 gcactttaag aaacaaaaga ggctaatccc tgagagaacc gtttggaaat acttcgttca
      541 gctctgcagt gcactggacc acatgcattc tcgaagagtc atgcacagag atataaaacc
      601 agctaatgtg ttcattacag ccactggggt agtaaaactc ggagaccttg ggcttggtcg
      661 gtttttcagc tccaaaacca cagctgcaca ttctttagtg ggtacacctt actacatgtc
      721 tccagagaga atacatgaaa atggatacaa cttcaagtct gacatctggt ctcttggctg
      781 tctgctatat gagatggctg cactgcagag tcctctctac ggcgacaaga tgaacttgta
      841 ttctctgtgt aagaagatag agcagtgtga ctacccgcct ctccc
//
