LOCUS       sequence-146583-         532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that serves as the PCR template to create genomic pdt1E 
            insertions. Plasmid contains the R6K origin of replication so needs 
            pir gene to replicate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECT1E
FEATURES             Location/Qualifiers
     source          1..532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    251..284
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cactggtgaa aagaaaaacc accctggctc ccaatacgca aaccgcctct ccccgcgcgt
       61 tggccgattc attaatgcaa ctggcacgac aggtttcccg actggaaagc gggcaggcgg
      121 cgaacaaaaa cgaagaaaac accaacgaag tgcccgacgg aatgctgaac gcgggccagg
      181 cgaaccgtct tcagttgtaa ccgcggagga gaaagtacca tgattccggg gatccgtcga
      241 cctgcagttc gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaagc
      301 tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa
      361 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca
      421 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag
      481 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg gg
//
