LOCUS       sequence-146731-         484 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for 
            expression control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Paquet D, Kwart D, Chen A, Sproul A, Jacob S, Teo S, Olsen KM, Gregg
            A, Noggle S, Tessier-Lavigne M
  TITLE     Efficient introduction of specific homozygous and heterozygous 
            mutations using CRISPR/Cas9.
  JOURNAL   Nature. 2016 May 5;533(7601):125-9. doi: 10.1038/nature17664. Epub 
            2016 Apr 27.
  PUBMED    27120160
REFERENCE   2  (bases 1 to 484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17664"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "125-9"
COMMENT     Sequence Label: pCas9-VRER_2A_GFP
FEATURES             Location/Qualifiers
     source          1..484
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agaagtactc cattgggctc gatatcggca caaacagcgt cggctgggcc gtcattacgg
       61 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
      121 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc gaagccacgc
      181 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
      241 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
      301 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
      361 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
      421 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
      481 aatt
//
