LOCUS       sequence-146780-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW101ParsR-gfp
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..35
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      479..550
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      566..593
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tttacagcta gctcagtcct tggtattatg ctagctccta gggtcacaca ggaaagtacc
       61 atatgtcatt tctgttaccc atccaattgt tcaaaattct tgctgatgaa acccgtctgg
      121 gcatcgtttt actgctcagc gaactgggag agttatgcgt ctgcgatctc tgcactgctc
      181 tcgaccagtc gcagcccaag atctcccgcc acctggcatt gctgcgtgaa agcgggctat
      241 tgctggaccg caagcaaggt aagtgggttc attaccgctt atcaccgcat attccagcat
      301 gggcggcgaa aattattgat gaggcctggc gatgtgaaca ggaaaaggtt caggcgattg
      361 tccgcaacct ggctcgacaa aactgttccg gggacagtaa gaacatttgc agttaaaaat
      421 ttagctaaac acacatgaat tttcagatgt gttttatccg ggtactagag ccaggcatca
      481 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      541 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      601 actagagcca actcaaaatt cacacctatt accttcctct gcacttacac attcgttaag
      661 tcatatatgt ttttgactta tccgcttcga agagagacac tacctgcaa
//
