LOCUS       sequence-146849-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PG1424PGA
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    73..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             145..162
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             187..207
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgccggccc gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg
       61 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg tgtagatctg
      181 ggtaccgaga acctgtactt ccaatccatg aatccccctg caggtcctgt gcgtgctatc
      241 gctgagtacg aacgctctgc agccgttttg gtacgctacc cgttcggtat cccgatggaa
      301 ttgatcaaag agctggccaa gaacgacaag gtgattacca ttgtggcgag tgaaagccaa
      361 aaaaacaccg ttataaccca gtacacccaa agcggtgtga atctctctaa ttgcgatttc
      421 atcattgcga aaactgactc ttactggaca cgcgactata ccggttggtt cgcaatgtac
      481 gatacgaaca aagtaggtct cgtggacttt atttataacc gccctcgtcc taacgatgat
      541 gaattcccca aatacgaagc acaatatctg ggcatcgaga tgttcgggat gaagctcaag
      601 cagaccggtg gcaactacat gacggacgga tatggatccg ctgtgcagtc acatatcgca
      661 tatacggaga actcctctct gtctcaagct caagtaaatc aaaagatgaa agactatctc
      721 ggcatcacac atcatgatgt ggtacaagat ccgaacggcg aatatatcaa ccatgtggac
      781 tgttggggca agtatttggc accgaacaaa atcctcatca ggaaagtgcc tgacaatcac
      841 cctcagcacc aagccctgga agatatggca gcctact
//
