LOCUS       sequence-146958-        4393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Wischnewski F, Friese O, Pantel K, Schwarzenbach H
  TITLE     Methyl-CpG binding domain proteins and their involvement in the 
            regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 gene 
            promoters.
  JOURNAL   Mol Cancer Res. 2007 Jul;5(7):749-59.
  PUBMED    17634428
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res."; date: "2007-07"; volume: "5(7)"; pages: "749-59"
COMMENT     Sequence Label: MAGE-A1Pro pGL4basic
FEATURES             Location/Qualifiers
     source          1..4393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      245..254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             251..1903
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1947..2068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1958..1977)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2012..2031
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2316..2333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2487..3075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2567..2586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3275..4066,4067..4135))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4240..4288
                     /note="synthetic polyadenylation signal"
     misc_feature    4302..4393
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4342..4361
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccg ttcccgccag gaaacatccg ggtgcccgga tgtgacgcca
       61 ctgacttgcg cattgtgggg cagagagaag cgaggtttcc attctgaggg acggcgtaga
      121 gttcggccga aggagcctga cccaggctct gtgaggaggc aaggtgagag gctgagggag
      181 gactgaggac cccgccactc caaatagaga gccccaagct tggcaatccg gtactgttgg
      241 taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact
      301 cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc
      361 cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt
      421 cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg
      481 gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt
      541 catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag
      601 catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct
      661 caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga
      721 ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa
      781 cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa
      841 cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt
      901 ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat
      961 cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat
     1021 ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt
     1081 gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa
     1141 gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc
     1201 gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg
     1261 ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga
     1321 caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga
     1381 caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat
     1441 catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg
     1501 gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg
     1561 gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat
     1621 cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc
     1681 cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga
     1741 gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt
     1801 gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga
     1861 gattctcatt aaggccaaga agggcggcaa gatcgccgtg taataattct agagtcgggg
     1921 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     1981 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2041 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2101 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     2161 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     2221 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     2281 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2341 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2401 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2461 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2521 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2581 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2641 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2701 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2761 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2821 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2881 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2941 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3001 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3061 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3121 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3181 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3241 gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac
     3301 cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga
     3361 tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc
     3421 cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa
     3481 gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta
     3541 gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg
     3601 tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc
     3661 gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg
     3721 ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt
     3781 ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt
     3841 cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca
     3901 acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc
     3961 ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac
     4021 ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca
     4081 agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg
     4141 tcctttttca atattattga agcatttatc agggttacta gtacgtctct caaggataag
     4201 taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca
     4261 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
     4321 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
     4381 agaacatttc tct
//
