LOCUS       sequence-147104-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for mammalian expression of HCMV protein UL136. Expresses 
            only the 26kda isoform of UL136. Also expresses EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F
  TITLE     Complex expression of the UL136 gene of human cytomegalovirus 
            results in multiple protein isoforms with unique roles in 
            replication.
  JOURNAL   J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 
            2014 Oct 8.
  PUBMED    25297993
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02711-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "24"; pages: "14412-25"
COMMENT     Sequence Label: pCIG1_UL136_26kDa_Myc
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..32
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             643..666
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             679..702
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
       61 acctccatag aagattctag agctagcgaa ttcaccatga ccgcggtgtt tcacgttatc
      121 tgtgccgttt tgcttacgct tgcgattgcg gccatcggcg cgctcatcgc gtacttaaga
      181 tattaccacc aggacagttg gcgagacgcg ctccacgatc tattttgcgg ctgtcattat
      241 cctgagaagt gccgtcggca ccacgagcgg cagagaagca gacggcgagc cgcggatgtg
      301 cccgacccgg aactcggcga cccggcccgc cggccgttga acggggccat gtactacggc
      361 agcggctgtc gcttcgacac ggtggaaatg gtggacgaga cgagacccgc gccgccggcg
      421 ctgtcatcgc ccgaaaccgg cgacgatagc aacgacgacg cggttgccgg cggaggtgct
      481 ggcggggtaa catcatccgc gactcgtacg acgtcgtcga acgcgctgct gccagaatgg
      541 atggatgcgg tacatgtggc ggtccaagcc gccgttcaag cgaccgtgca agtaagtggc
      601 ccgcgggaga acgccgtatc tcccgctacg ggcggtggcg gttggagcca cccgcagttc
      661 gaaaaaggcg gtggcggttg gagccacccg cagttcgaaa aataaggatc caattccgcc
//
