LOCUS       sequence-147117-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, 
            Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-B9D2-N-18
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      619..628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(679..730)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(708..728)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tacggagttt gatcctcatg tctggcttaa tcgcactcac catggtggcg accggtggat
       61 cccgggcccg cggtaccgtc gactgcagaa ttcccacaca ctccactcca tagcgatcga
      121 agtggcgcag cagcaggccg ataccaaggt gcactgtgcc acccgcggcc gtgtgcaggc
      181 ggtagcggtc agccccgctg tagatggtat ctgcgtgcag cagctgtggc ccaccaccca
      241 cgaaagcccg tgctagctgc tccctccaac tgcccagggg cctccatgta gggcagtcca
      301 gctgatgagt gcctgggctg ctaggcacat ggcaaaagcc atagccagca agctggcagc
      361 ggccaaagct gtcctgggac cacacctgga gatggagtcg aggccaacct tggagacctt
      421 tagtagcgaa gtgcaggtca atggggtggg accagtaagc catgtcccct atctggggtg
      481 tgtctacctg tgtctggcct tctcgtacac ctgagaggag cttccatgcg gcccctgtgt
      541 ggatgcccca cttgcaaaag aggctgcttt ccgagaaacc ggtggcccct atgatctgcc
      601 cgatcacgtg cacttcagcc atggtggcct cgagatctga gtccggtagc gctagcggat
      661 ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc
      721 catttgcgtc aatggg
//
