LOCUS       sequence-147197-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Sabourin LA, Rudnicki MA
  TITLE     The molecular regulation of myogenesis.
  JOURNAL   Clin Genet. 2000 Jan . 57(1):16-25.
  PUBMED    10733231
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Clin Genet.
            2000 Jan . 57(1):16-25."
COMMENT     Sequence Label: EMSV-MRF4 (puro)
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(13..513)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(62..81)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     455..478
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(572..591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(573..591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(598..615)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(598..614)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(607..629)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atggatgcag gtcgaaaggc ccggaagatg aggaagagga gaacagcgcg gcagacgtgc
       61 gcttttgaag cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc
      121 tgagcccgcc cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag
      181 cgaaggagca aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag
      241 cggtgctgtc catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc
      301 acgacgcgag ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg
      361 cgaaggggcc accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc
      421 gaggccagag gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc
      481 cagactgcct tgggaaaagc gcctccccta cccggtagaa tttcgaggtc gactctagag
      541 gatccccggg taccgagctc gaattaattc gccctatagt gagtcgtatt acaattcact
      601 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      661 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      721 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cc
//
