LOCUS       sequence-147261-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the 
            pyrin domain) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-Flag-NLRP3 1-90
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(263..286)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(326..345)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(327..345)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(390..593)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(419..439)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cccagaggtc tcgcctgttg atcgcagcaa agatccacac agccatggcc caggccttct
       61 cctcaccatt gaagtcaatc atgagtgtgg ctagatccaa gtgatctgcc ttctccatct
      121 ggcccctggg gactgggata cagcctttct cgggcgggta atcttccaaa tgcattttga
      181 atttcttgag gtccacatct tcaaggtcct ctagatactg agccagcttg caacggacac
      241 tcgtcatgaa ttcgatatcg attttatcgt catcgtcttt gtagtccatg gtgggcgatc
      301 cgagctcggt accaagcttg ggtctcccta tagtgagtcg tattaatttc gataagccag
      361 taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc ccaccgtaca
      421 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
      481 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
      541 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
      601 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
      661 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
      721 atatgataca ctt
//
