LOCUS       sequence-147325-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L1U1H08 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L1U1H08
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(23..66)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    69..88
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      181..238
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(262..279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(513..532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
       61 gatttctgga attcgcggcc gcttctagaa tctagagacg catatgggtc tcatcgaccc
      121 gcatgttcgc atgcgggttt tttttttgtt agagaccgca tgccgtctca ttagctgcag
      181 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
      241 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      301 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      361 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      421 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      601 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      661 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      721 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgcc
//
