LOCUS       sequence-147334-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modification of CYP2C9 and 2C19 s for bacterial expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     Goldstein CYP2C9 and CYP2C9 in pOri+ for bacterial expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CYP2C9 in vector pCW ori+
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(139..144,145..162,163..169)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    177..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(248..253,254..269,270..276)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    284..300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(343..348,349..364,365..371)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    379..395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgggcgctat catgccatac cgcgaaaggt tttgcaccat tcgatggtgt cctggcacga
       61 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac
      121 tcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggaattgt
      181 gagcggataa caatttcaca caggaaacag gatcgatcca tcgatgagct tactccccat
      241 ccccctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa
      301 tttcacacag gaaacaggat cagcttactc cccatccccc tgttgacaat taatcatcgg
      361 ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac aggatccatc
      421 gatgcttagg aggtcatatg gctctgttat tagcagtttt tctctgtctc tcatgtttgc
      481 ttctcctttc actctggaga cagagctctg ggagaggaaa actccctcct ggccccactc
      541 ctctcccagt gattggaaat atcctacaga taggtattaa ggacatcagc aaatccttaa
      601 ccaatctctc aaaggtctat ggccctgtgt tcactctgta ttttggcctg aaacccatag
      661 tggtgctgca tggatatgaa gcagtgaagg aagccctgat tgatcttgga gaggagtttt
      721 ctggaagagg cattttccca ctggctgaaa gagctaacag aggatttgga attgttttca
      781 gcaatggaaa gaaatggaag gagatccggc gtttctccct catgacgctg cggaattttg
      841 ggatggggaa gaggagcatt gaggaccgtg ttcaagagga agcccgctgc cttgtgga
//
