LOCUS       sequence-147396-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the expression of Luciferase and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  TITLE     Saradhi Mallampati (Pre-published)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MIG-Luciferase-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggcgccggaa ttagatctct cgaggttaac gaattcgcca ccatggaaga tgccaaaaac
       61 attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg
      121 cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat
      181 atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct
      241 atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg
      301 cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac
      361 gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta
      421 ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata
      481 caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc
      541 ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc
      601 gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag
      661 ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcc
//
