LOCUS       sequence-147531-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Sen GL, Webster DE, Barragan DI, Chang HY, Khavari PA
  TITLE     Control of differentiation in a self-renewing mammalian tissue by 
            the histone demethylase JMJD3.
  JOURNAL   Genes Dev. 2008 Jul 15. 22(14):1865-70.
  PUBMED    18628393
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Jul 15. 22(14):1865-70."
COMMENT     Sequence Label: MSCV JMJD3
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..71
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(571..582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctgc caccatggac tacaaggatg
       61 acgatgacaa gctcgatgga ggatacccat acgatgttcc agattacgct gctcgaggtt
      121 atcaaacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc ggatccatgc
      181 atcgggcagt ggatcctcca ggggcccgcg ctgcacggga agcctttgcc cttgggggcc
      241 tgagctgtgc tggggcctgg agctcctgcc cgcctcatcc ccctcctcgt agcgcatggc
      301 tgcctggagg cagatgctca gccagcattg ggcagccccc gcttcctgct cccctacccc
      361 cttcacatgg cagtagttct gggcacccca gcaaaccata ttatgctcca ggggcgccca
      421 ctccaagacc cctccatggg aagctggaat ccctgcatgg ctgtgtgcag gcattgctcc
      481 gggagccagc ccagccaggg ctttgggaac agcttgggca actgtacgag tcagagcacg
      541 atagtgagga ggccacacgc tgctaccaca gcgcccttcg atacggagga agcttcgctg
      601 agctggggcc ccgca
//
