LOCUS       sequence-147534-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Takanaga H, Chaudhuri B, Frommer WB
  TITLE     GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 
            cells identified by a high sensitivity intramolecular FRET glucose 
            sensor.
  JOURNAL   Biochim Biophys Acta. 2008 Apr . 1778(4):1091-9.
  PUBMED    18177733
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2008 Apr . 1778(4):1091-9."
COMMENT     Sequence Label: GLUT1-eGFP/pcDNA-DEST47
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagtt aagcttgatc aaacaagttt gtacaaaaaa gcaggctccg
      121 cggccgcccc cttcaccatg gagcccagca gcaagaagct gacgggtcgc ctcatgctgg
      181 ccgtgggagg agcagtgctt ggctccctgc agtttggcta caacactgga gtcatcaatg
      241 ccccccagaa ggtgatcgag gagttctaca accagacatg ggtccaccgc tatggggaga
      301 gcatcctgcc caccacgctc accacgctct ggtccctctc agtggccatc ttttctgttg
      361 ggggcatgat tggctccttc tctgtgggcc ttttcgttaa ccgctttggc cggcggaatt
      421 caatgctgat gatgaacctg ctggccttcg tgtccgccgt gctcatgggc ttctcgaaac
      481 tgggcaagtc ctttgagatg ctgatcctgg gccgcttcat catcggtgtg tactgcggcc
      541 tgaccacagg cttcgtgccc atgtatgtgg gtgaagtgtc ac
//
