LOCUS       sequence-147535-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Takanaga H, Chaudhuri B, Frommer WB
  TITLE     GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 
            cells identified by a high sensitivity intramolecular FRET glucose 
            sensor.
  JOURNAL   Biochim Biophys Acta. 2008 Apr . 1778(4):1091-9.
  PUBMED    18177733
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2008 Apr . 1778(4):1091-9."
COMMENT     Sequence Label: GLUT1-eGFP/pcDNA-DEST47
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(34..54)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     649..677
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    721..745
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctcatcca tgccatgtgt aatcccagca gcagttacaa actcaagaag gaccatgtgg
       61 tcacgctttt cgttgggatc tttcgaaagg gcagattgtg tcgacaggta atggttgtct
      121 ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga
      181 acggatccat cttcaatgtt gtggcgaatt ttgaagttag ctttgattcc attcttttgt
      241 ttgtctgccg tgatgtatac attgtgtgag ttatagttgt actcgagttt gtgtccgaga
      301 atgtttccat cttctttaaa atcaatacct tttaactcga tacgattaac aagggtatca
      361 ccttcaaact tgacttcagc acgcgtcttg tagttcccgt catctttgaa agatatagtg
      421 cgttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg tttcatatga
      481 tccggataac gggaaaagca ttgaacacca taagagaaag tagtgacaag tgttggccat
      541 ggaacaggta gttttccagt agtgcaaata aatttaaggg taagctttcc gtatgtagca
      601 tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc atctaattca
      661 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgctagccat tctagatcga
      721 accactttgt acaagaaagc tgggtcggcg cgcccaccct tcacttggga atcagccccc
      781 aggggatgga acagctcctc gggtgtcttg tcactttggc tggctccccc ctgccggaag
      841 ccggaagcga tctcatcgaa ggtccggcct ttagtctcag gaactttgaa gtaggtgaag
      901 atgaagaaca gaaccaggag cacagtgaag atgatgaaga cgtagggacc acacagttgc
      961 tccacatact ggaagcacat gcc
//
