LOCUS       sequence-147704-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses human optimized S. pyogenes dCas9 and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: pLV hUbC-dCas9-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             129..194
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             201..221
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa
       61 attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc gactctagag
      121 ccaccatgga ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg
      181 atgacgatga caagatggcc cccaagaaga agaggaaggt gggccgcgga atggacaaga
      241 agtactccat tgggctcgcc atcggcacaa acagcgtcgg ctgggccgtc attacggacg
      301 agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa
      361 agaagaacct cattggcgcc ctcctgttcg actccgggga aaccgccgaa gccacgcggc
      421 tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg
      481 agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt
      541 cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg
      601 acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagcttgtag
      661 acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat atgatcaaat
      721 ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac
      781 tctttatcca actggttcag acttacaatc agctt
//
