LOCUS       sequence-147792-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of SLC24A2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Visser F, Valsecchi V, Annunziato L, Lytton J
  TITLE     Exchangers NCKX2, NCKX3, and NCKX4: identification of Thr-551 as a 
            key residue in defining the apparent K(+) affinity of NCKX2.
  JOURNAL   J Biol Chem. 2007 Feb 16;282(7):4453-62. Epub 2006 Dec 15.
  PUBMED    17172467
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-02-16"; volume: "282(7)"; pages: "4453-62. Epub 
            2006 Dec 15"
COMMENT     Sequence Label: Human NCKX2-AS-short-FlagTag (pcDNA3.1+)
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             377..400
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccatggatct
      121 gcaacaaagc accaccatca cttccctaga gaaatggtgt ttggatgagt cactgtctgg
      181 ctgcagaaga cattatagtg tcaagaaaaa actgaagtta attcgagtct taggcctttt
      241 catgggtctg gtagccatta gcactgtctc attttcaatc agtgcctttt ctgagacaga
      301 tacacagagc acaggagagg ccagtgttgt aagtggccct agggtagcac agggttacca
      361 tcagagaact ctcttagatt acaaggacga cgatgacaag tatactccac agccacctct
      421 ttctaaggaa ggcgagtctg agaatagtac agatcacgcc caaggagact acccgaaaga
      481 catcttttcc cttgaggaga gaagaaaagg tgcgatcatt ctgcatgtca ttggaatgat
      541 ctacatgttc atagccttag ccattgtctg tgatgagttc tttgttcctt ctttgactgt
      601 catcactgaa aaactgggca tctctgatga tgtggctgga gccaccttca tggctgcagg
      661 agggtcagcc ccagaacttt tcacatctct cataggggta tttatcgctc acagcaacgt
      721 tggcataggc acaattgtag gttcagcagt attcaacatc ctctttgtta ttggcatgtg
      781 tgctctgttt tctagagaaa tcttaaacct gacatggtgg ccgctctttc gagatgtgtc
      841 tttctacatt gttgacttga tcatgctgat catatttttc ctggataatg tcatcatgtg
      901 gtgggaaagc ttgcttctct taacagctta tttttgctat gtggttttca tgaaattcaa
      961 cgtccaagta gaaaaatggg tgaagcaaat gataaa
//
