LOCUS       sequence-147795-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of SLC24A2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Visser F, Valsecchi V, Annunziato L, Lytton J
  TITLE     Exchangers NCKX2, NCKX3, and NCKX4: identification of Thr-551 as a 
            key residue in defining the apparent K(+) affinity of NCKX2.
  JOURNAL   J Biol Chem. 2007 Feb 16;282(7):4453-62. Epub 2006 Dec 15.
  PUBMED    17172467
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-02-16"; volume: "282(7)"; pages: "4453-62. Epub 
            2006 Dec 15"
COMMENT     Sequence Label: Human NCKX2-AS-long-FlagTag (pcDNA3.1+)
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             376..399
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaata gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttggtac catggatctg
      121 caacaaagca ccaccatcac ttccctagag aaatggtgtt tggatgagtc actgtctggc
      181 tgcagaagac attatagtgt caagaaaaaa ctgaagttaa ttcgagtctt aggccttttc
      241 atgggtctgg tagccattag cactgtctca ttttcaatca gtgccttttc tgagacagat
      301 acacagagca caggagaggc cagtgttgta agtggcccta gggtagcaca gggttaccat
      361 cagagaactc tcttagatta caaggacgac gatgacaagt atactccaca gccacctctt
      421 tctaaggaag gcgagtctga gaatagtaca gatcacgccc aaggagacta cccgaaagac
      481 atcttttccc ttgaggagag aagaaaaggt gcgatcattc tgcatgtcat tggaatgatc
      541 tacatgttca tagccttagc cattgtctgt gatgagttct ttgttccttc tttgactgtc
      601 atcactgaaa aactgggcat ctctgatgat gtggctggag ccaccttcat ggctgcagga
      661 gggtcagccc cagaactttt cacatctctc ataggggtat ttatcgctca cagcaacgtt
      721 ggcataggca caattgtagg ttcagcagta ttcaacatcc tctttgttat tggcatgtgt
      781 gctctgtttt ctagagaaat cttaaacctg acatggtggc cgctctttcg agatgtgtct
      841 ttctacattg ttgacttgat catgctgatc atatttttcc tggataatgt catcatgtgg
      901 tgggaaagct tgcttctctt aacagcttat ttttgctatg tggttttcat gaaattcaac
      961 gtccaagtag aa
//
