LOCUS       sequence-147965-         598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Lee BI, Kwak JE, Suh SW
  TITLE     Crystal structure of the type II 3-dehydroquinase from Helicobacter 
            pylori.
  JOURNAL   Proteins. 2003 Jun 1. 51(4):616-7.
  PUBMED    12784220
REFERENCE   2  (bases 1 to 598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2003 Jun 1. 51(4):616-7."
COMMENT     Sequence Label: pET-21a(+)-AroQ
FEATURES             Location/Qualifiers
     source          1..598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    64..88
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             103..125
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat
       61 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
      121 ggagatatac atatgaaaat tttagtgatt caagggccta atttaaacat gttaggacac
      181 agagacccaa ggctttatgg tatggtaacc ttagaccaaa tccatgaaat catgcaaact
      241 ttcgtgaaac aaggcaattt agatgtggaa ttagagtttt ttcaaactaa ttttgagggc
      301 gaaatcattg ataaaatcca agagagcgtg ggcagcgatt atgaagggat catcattaac
      361 cctggagcgt tttcgcacac ttctattgcg attgcagatg cgatcatgct agcgggcaaa
      421 cccgttattg aagtgcatct cactaacatt caagccagag aggaattcag gaaaaattct
      481 tacactggag cggcttgtgg aggcgtgatc atgggatttg gcccgcttgg ctacaacatg
      541 gctttaatgg cgatggtcaa tattttagcc gaaatgaaag cgttccaaga agcccaaa
//
