LOCUS       sequence-148020-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors
            with a constitutive mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim 
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using 
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. 
            Epub 2016 Jan 28.
  PUBMED    26830878
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date: 
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
COMMENT     Sequence Label: pHR_Gal4UAS_tBFP_PGK_mCherry
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..699
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
       61 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      121 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
      181 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
      241 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      301 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
      361 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
      421 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
      481 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
      541 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
      601 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
      661 tactgcgacc tccctagcaa actggggcac aagcttaat
//
