LOCUS       sequence-148067-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive expression of gfp-pdt3B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pZE27GFP3B
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(214..300)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(467..487)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      181 ccgcagccga acgccctagg tctagggcgg cggatttgtc ctactcagga gagcgttcac
      241 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      301 atgcctctag cacgcgtacc atgggatccc ccatcaagct tattaaactc gtcttctgtt
      361 cgcctggccc gcgttcagca tgatcgcgcg cacttcgttg gtgttttctt cgtttttgtt
      421 cgccgctttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
      481 aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      541 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      601 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      661 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      721 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      781 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      841 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaa
//
