LOCUS       sequence-148172-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against PICK1 as well as the marker Venus 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Anggono V, Clem RL, Huganir RL
  TITLE     PICK1 loss of function occludes homeostatic synaptic scaling.
  JOURNAL   J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 
            10.1523/JNEUROSCI.5633-10.2011.
  PUBMED    21307255
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.5633-10"; journalName: "J Neurosci"; date: 
            "2011-02-9- 9"; volume: "31"; issue: "6"; pages: "2188-96"
COMMENT     Sequence Label: pVenus-PICK1-shRNA
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(128..133,134..151,152..158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    166..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     171..193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     190..206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     190..206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        227..245
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(343..557)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(403..422)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       61 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      181 aatttcacac aggaaacagc tatgaccatg attacgccaa gctcgaaatt aaccctcact
      241 aaagggaaca aaagctggta ccgggccccc cctcgagaaa aaagcctcac catcaagaag
      301 tacctctctt gaaggtactt cttgatggtg aggcggggat ctgtggtctc atacagaact
      361 tataagattc ccaaatccaa agacatttca cgtttatggt gatttcccag aacacatagc
      421 gacatgcaaa tattgcaggg cgccactccc ctgtccctca cagccatctt cctgccaggg
      481 cgcacgcgcg ctgggtgttc ccgcctagtg acactgggcc cgcgattcct tggagcgggt
      541 tgatgacgtc agcgttcgaa ttcctgcagc cctcgaccgc caattcaata tggcgtatat
      601 ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat
      661 ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata tggcggactt
      721 ggcaccatgc caattcaata tgg
//
