LOCUS       sequence-148173-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against PICK1 as well as the marker Venus 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Anggono V, Clem RL, Huganir RL
  TITLE     PICK1 loss of function occludes homeostatic synaptic scaling.
  JOURNAL   J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 
            10.1523/JNEUROSCI.5633-10.2011.
  PUBMED    21307255
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.5633-10"; journalName: "J Neurosci"; date: 
            "2011-02-9- 9"; volume: "31"; issue: "6"; pages: "2188-96"
COMMENT     Sequence Label: pVenus-PICK1-shRNA
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(69..71,72..74,75..788)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(114..135)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(375..394)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     722..743
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    826..960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(850..869)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     904..923
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 aattcaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      121 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      181 ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct
      241 ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc
      301 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      361 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      421 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      481 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc
      541 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
      601 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      661 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      721 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      781 acaagtaagt cgagcctcta gaactatagt gagtcgtatt acgtagatcc agacatgata
      841 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      901 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      961 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
//
