LOCUS       sequence-148179-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Anggono V, Koc-Schmitz Y, Widagdo J, Kormann J, Quan A, Chen CM, 
            Robinson PJ, Choi SY, Linden DJ, Plomann M, Huganir RL
  TITLE     PICK1 interacts with PACSIN to regulate AMPA receptor 
            internalization and cerebellar long-term depression.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13976-81. doi: 
            10.1073/pnas.1312467110. Epub 2013 Aug 5.
  PUBMED    23918399
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1312467110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-20- 20"; volume: "110"; issue: "34"; pages: 
            "13976-81"
COMMENT     Sequence Label: pRK5-H1-shRNA-CMV-HA-PACSIN1
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..49
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(166..183)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(390..604)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(450..469)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(624..640)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(624..640)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(672..691)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(673..691)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(833..852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
       61 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      121 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tttaaccctc ataaagggaa
      301 caaaagctgg taccgggccc cccctcgaga aaaaagcgcc agctcatcga gaaatctctt
      361 aatttctcga tgagctggcg cggggatctg tggtctcata cagaacttat aagattccca
      421 aatccaaaga catttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat
      481 tgcagggcgc cactcccctg tccctcacag ccatcttcct gccagggcgc acgcgcgctg
      541 ggtgttcccg cctagtgaca ctgggcccgc gattccttgg agcgggttga tgacgtcagc
      601 gttcgaattc ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga
      661 gctccaattc gccctatagt gagtcgtatt aacatgtgag caaaaggcca gcaaaaggcc
      721 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      781 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      841 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      901 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      961 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtg
//
