LOCUS       sequence-148185-       11885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ERG after doxycycline administration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Doulatov S, Vo LT, Chou SS, Kim PG, Arora N, Li H, Hadland BK, 
            Bernstein ID, Collins JJ, Zon LI, Daley GQ
  TITLE     Induction of multipotential hematopoietic progenitors from human 
            pluripotent stem cells via respecification of lineage-restricted 
            precursors.
  JOURNAL   Cell Stem Cell. 2013 Oct 3;13(4):459-70. doi: 
            10.1016/j.stem.2013.09.002.
  PUBMED    24094326
REFERENCE   2  (bases 1 to 11885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.09"; journalName: "Cell Stem Cell"; date: 
            "2013-10-3- 3"; volume: "13"; issue: "4"; pages: "459-70"
COMMENT     Sequence Label: pINDUCER21-ERG
FEATURES             Location/Qualifiers
     source          1..11885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    230..251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(266..271,272..289,290..296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    304..320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     309..331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     328..344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     328..344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     394..413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             605..1238
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1285..1410
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1907..2140
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2325..2369
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2662..2779
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2834..2852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2876..2894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2918..2936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2960..2978
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3002..3020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3053..3091
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3088..3112
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3221..3245
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4719..4743)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4796..4822
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5042..6220
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5273..6211
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6168..6188
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    6290..6323
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(6330..6947,6948..6953,6954..6989,6990..6992,6993..7028,
                     7029..7031,7032..7067,7068..7076)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7128..7701
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7295..7312)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7522..7541
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7717..7719,7720..7722,7723..8436)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7717..7719,7720..7722,7723..8433)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7762..7783)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8023..8042)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8370..8391
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8572..8591
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8879..9467
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8932..8952)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9350..9361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9542..9775
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9894..9912)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9980..10084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10085..10153,10154..10945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10303..10322)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11116..11704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11605..11624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11858..11875
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
       61 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
      121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
      181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
      361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      421 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
      481 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
      541 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
      601 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
      661 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      721 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      781 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      841 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      901 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      961 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     1021 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     1081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1261 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1321 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1381 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1441 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1501 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1561 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1621 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1681 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1741 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1801 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1861 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1921 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1981 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2041 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2101 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2161 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2221 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2281 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2341 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2401 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2461 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2521 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2581 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2641 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2701 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2761 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2821 gatcctttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2881 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2941 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     3001 ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg
     3061 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     3121 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     3181 ccccgaacta gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc
     3241 aggcttggaa ggagttcgaa ccatggccag cactattaag gaagccttat cagttgtgag
     3301 tgaggaccag tcgttgtttg agtgtgccta cggaacgcca cacctggcta agacagagat
     3361 gaccgcgtcc tcctccagcg actatggaca gacttccaag atgagcccac gcgtccctca
     3421 gcaggattgg ctgtctcaac ccccagccag ggtcaccatc aaaatggaat gtaaccctag
     3481 ccaggtgaat ggctcaagga actctcctga tgaatgcagt gtggccaaag gcgggaagat
     3541 ggtgggcagc ccagacaccg ttgggatgaa ctacggcagc tacatggagg agaagcacat
     3601 gccaccccca aacatgacca cgaacgagcg cagagttatc gtgccagcag atcctacgct
     3661 atggagtaca gaccatgtgc ggcagtggct ggagtgggcg gtgaaagaat atggccttcc
     3721 agacgtcaac atcttgttat tccagaacat cgatgggaag gaactgtgca agatgaccaa
     3781 ggacgacttc cagaggctca cccccagcta caatgccgac atccttctct cacatctcca
     3841 ctacctcaga gagactcctc ttccacattt gacttcagat gatgttgata aagccttaca
     3901 aaactctcca cggttaatgc atgctagaaa cacagggggt gcagctttta ttttcccaaa
     3961 tacttcagta tatcctgaag ctacgcaaag aattacaact aggccagatt taccatatga
     4021 gccccccagg agatcagcct ggaccggtca cggccacccc acgccccagt cgaaagctgc
     4081 tcaaccatct ccttccacag tgcccaaaac tgaagaccag cgtcctcagt tagatcctta
     4141 tcagattctt ggaccaacaa gtagccgcct tgcaaatcca ggcagtggcc agatccagct
     4201 ttggcagttc ctcctggagc tcctgtcgga cagctccaac tccagctgca tcacctggga
     4261 aggcaccaac ggggagttca agatgacgga tcccgacgag gtggcccggc gctggggaga
     4321 gcggaagagc aaacccaaca tgaactacga taagctcagc cgcgccctcc gttactacta
     4381 tgacaagaac atcatgacca aggtccatgg gaagcgctac gcctacaagt tcgacttcca
     4441 cgggatcgcc caggccctcc agccccaccc cccggagtca tctctgtaca agtacccctc
     4501 agacctcccg tacatgggct cctatcacgc ccacccacag aagatgaact ttgtggcgcc
     4561 ccaccctcca gccctccccg tgacatcttc cagttttttt gctgccccaa acccatactg
     4621 gaattcacca actgggggta tataccccaa cactaggctc cccaccagcc atatgccttc
     4681 tcatctgggc acttactact agctcgagtg cggccgcaac ccagctttct tgtacaaagt
     4741 ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaataccc
     4801 atacgacgtc ccagactacg cttagtaatg attaattaaa ctagaaattc taccgggtag
     4861 gggaggcgct tttcccaagg cagtctggag taacccaccc aagatctggc ctccgcgccg
     4921 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agccgcgtaa gatctggcct
     4981 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cctagtcgtg
     5041 aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     5101 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     5161 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     5221 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     5281 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     5341 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     5401 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     5461 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     5521 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     5581 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     5641 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     5701 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     5761 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     5821 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     5881 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     5941 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     6001 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     6061 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     6121 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     6181 agtggttcaa agtttttttc ttccatttca ggtgtcgtga agcggccgcc atgctagtaa
     6241 attgtccgct aaattctggc cgtttttggc ttttttgtta gacggatcca taacttcgta
     6301 taatgtatgc tatacgaagt tatgccacca tgtctagact ggacaagagc aaagtcataa
     6361 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
     6421 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     6481 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
     6541 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
     6601 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     6661 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     6721 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     6781 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     6841 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     6901 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
     6961 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     7021 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa
     7081 gtaaggatcc gcggccgcac tagaggaatt ccgcccctct cccccccccc ctctccctcc
     7141 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     7201 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     7261 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     7321 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     7381 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     7441 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     7501 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     7561 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     7621 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     7681 ctttgaaaaa cacgatgata agcttaccgg tccaccatgg tgagcaaggg cgaggagctg
     7741 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     7801 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     7861 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     7921 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     7981 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     8041 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     8101 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     8161 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     8221 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     8281 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     8341 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     8401 gggatcactc tcggcatgga cgagctgtac aagtaagcat gcccgacggc gaggatctcg
     8461 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     8521 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     8581 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     8641 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     8701 gatgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     8761 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     8821 gcaattgttg ttgtttagtc cctcccaatt cgatatcaag cttatcgcga tagatcctaa
     8881 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     8941 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     9001 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     9061 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     9121 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     9181 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     9241 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     9301 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     9361 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     9421 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     9481 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     9541 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     9601 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9661 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9721 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     9781 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     9841 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     9901 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     9961 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    10021 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    10081 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    10141 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    10201 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    10261 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    10321 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    10381 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    10441 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    10501 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    10561 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    10621 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    10681 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    10741 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    10801 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    10861 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    10921 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    10981 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    11041 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    11101 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    11161 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    11221 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    11281 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    11341 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    11401 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    11461 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    11521 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    11581 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    11641 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    11701 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    11761 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    11821 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    11881 ggaag
//
