LOCUS       sequence-148188-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ffDronpa E. coli strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Moeyaert B, Nguyen Bich N, De Zitter E, Rocha S, Clays K, Mizuno H, 
            van Meervelt L, Hofkens J, Dedecker P
  TITLE     Green-to-red photoconvertible Dronpa mutant for multimodal 
            super-resolution fluorescence microscopy.
  JOURNAL   ACS Nano. 2014 Feb 25;8(2):1664-73. doi: 10.1021/nn4060144. Epub 
            2014 Jan 21.
  PUBMED    24410188
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/nn4060144";
            journalName: "ACS Nano"; date: "2014-02-25- 25"; volume: "8"; issue:
            "2"; pages: "1664-73"
COMMENT     Sequence Label: pRSETb-ffDronpa
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             50..82
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(86..94,95..109)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             116..790
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTTAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALSL
                     EGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPRQ
                     AK"
ORIGIN
        1 agaaggagat atacatatgc ggggttctca tcatcatcat catcatggta tggctagcat
       61 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgatgag
      121 tgtgattaaa ccagacatga agatcaagct gcgtatggaa ggcgctgtaa atggacaccc
      181 gttcgcgatt gaaggagttg gccttgggaa gcctttcgag ggaaaacaga gtatggacct
      241 taaagtcaaa gaaggcggac ctctgccttt cgcctatgac atcttgacaa ctgcgttctg
      301 ttacggcaac agggtattcg ccaaataccc agaaaatata gtagactatt tcaagcagtc
      361 gtttcctgag ggctactctt gggaacgaag catgaattac gaagacgggg gcatttgtaa
      421 cgcgacaaac gacataaccc tggatggtga ctgttatatc tatgaaattc gatttgatgg
      481 tgtgaacttt cctgccaatg gtccagttat gcagaagagg actgtgaaat gggagccatc
      541 cactgagaaa ttgtatgtgc gtgatggagt gctgaagggt gatgttaaca tggctctgtc
      601 gcttgaagga ggtggccatt accgatgtga cttcaaaact acttataaag ctaagaaggt
      661 tgtccagttg ccagactatc actttgtgga ccaccacatt gagattaaaa gccacgacaa
      721 agattacagt aatgttaatc tgcatgagca tgccgaagcg cattctgagc tgccgaggca
      781 ggccaagtaa gaattcgaa
//
