LOCUS       sequence-148203-       11398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11398)
  AUTHORS   Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM
  TITLE     Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is
            necessary for its activation by amino acids.
  JOURNAL   Cell. 2010 Apr 16. 141(2):290-303.
  PUBMED    20381137
REFERENCE   2  (bases 1 to 11398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 16. 141(2):290-303."
COMMENT     Sequence Label: pLJM1 Flag Raptor
FEATURES             Location/Qualifiers
     source          1..11398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        288..514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             515..695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    742..867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1360..1593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1778..1822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2120..2140
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2429..2632
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2583..2603
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2673..2696
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6779..6896
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6945..7455
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7374..7392
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7477..8076
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7477..7496)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7973..7993
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8204..8437
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8509..8630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8546..8565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8600..8619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8670..8805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8825..8844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8826..8844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8854..8871)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8854..8870)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8863..8885)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9012..9467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9099..9118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9309..9330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9493..9597
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9598..9666,9667..10458)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9816..9835)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10629..11217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11118..11137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11371..11388
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1981 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2041 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2101 agcggccgcc cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg
     2161 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     2221 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     2281 ttttaaaatg gactatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     2341 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     2401 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     2461 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     2521 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     2581 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     2641 gaaccgtcag atccgctagc gctaccggta tggactacaa ggatgacgat gacaagggtg
     2701 gtggtggtgg tgcgtcgact atggagtccg aaatgctgca atcgcctctt ctgggcctgg
     2761 gggaggaaga tgaggctgat cttacagact ggaacctacc tttggctttt atgaaaaaga
     2821 ggcactgtga gaaaattgaa ggctccaaat ccttagctca gagctggagg atgaaggatc
     2881 ggatgaagac agtcagtgtt gccttagttt tgtgcctgaa tgttggtgtg gaccctcccg
     2941 atgtggtgaa gaccacgccc tgtgcacgct tggaatgctg gatcgatcct ctgtcgatgg
     3001 gtcctcagaa agctctggaa accatcggtg caaatttaca gaagcagtac gagaactggc
     3061 agccaagggc ccggtacaag cagagccttg acccaactgt ggatgaagtc aagaagctct
     3121 gcacgtcctt acgtcgcaac gccaaggagg agcgagtcct ctttcactac aatggccacg
     3181 gggtgccccg gcccacagtc aacggggagg tctgggtctt caacaagaac tacacgcagt
     3241 acatccctct gtccatatat gacctgcaga cgtggatggg cagcccgtcg atcttcgtct
     3301 acgactgctc caatgctggc ttgatcgtca agtccttcaa gcagttcgca ctacagcggg
     3361 agcaggagct ggaggtagct gcaatcaacc caaatcaccc tcttgctcag atgcctttgc
     3421 ctccgtcgat gaaaaactgc atccagctgg cagcctgcga ggccaccgag ctgctgccca
     3481 tgatccccga cctcccggct gacctattca cctcctgcct caccaccccc atcaagatcg
     3541 ccctgcgctg gttttgcatg cagaaatgtg tcagtctggt gcctggcgtc acactggatt
     3601 tgatagaaaa gatccctggc cgcctgaacg acaggaggac gcccctgggt gaactgaact
     3661 ggatcttcac agccatcaca gacaccatcg cgtggaacgt gctcccccgg gatctcttcc
     3721 aaaagctctt cagacaggac ttgctggtgg ctagtctgtt tcgaaatttt ttattggcgg
     3781 aaaggattat gaggtcgtat aactgcactc ccgtcagcag cccgcgtctg ccgcccacgt
     3841 acatgcacgc catgtggcaa gcctgggacc tggctgttga catctgtctg tctcagctgc
     3901 cgacgatcat cgaggaaggc actgcgtttc ggcacagccc gttcttcgcc gagcagctga
     3961 ccgcattcca ggtgtggctc accatgggcg tggagaaccg aaacccaccc gaacagctgc
     4021 ccatcgtcct gcaggtgctg ttaagccaag tgcaccggct gagagcattg gacttgcttg
     4081 gaagattttt ggacctgggt ccctgggcag tgagcctggc cttgtctgtc ggcatcttcc
     4141 cctacgtgct gaagctgctc cagagctcgg cccgagagct gcggccactt ctcgttttca
     4201 tctgggccaa gatcctcgca gtggacagct cgtgccaggc ggacctcgtg aaggacaacg
     4261 gccacaagta cttcctgtcg gtcctggcgg acccctacat gccagctgaa caccggacca
     4321 tgacggcttt cattctcgcc gtgatcgtca acagctatca cacggggcag gaagcctgcc
     4381 ttcagggaaa cctcattgcc atctgcctgg agcagctcaa cgacccgcac cccttgctgc
     4441 gccagtgggt ggccatctgc ctcggcagga tctggcagaa cttcgactcg gcgaggtggt
     4501 gcggcgtgag ggacagcgct catgagaagc tctacagcct cctctccgac cccattcccg
     4561 aggtccgctg cgcagcggtc ttcgcccttg gcacgttcgt gggcaactct gcagagagga
     4621 cggaccactc caccaccatc gaccacaacg tggccatgat gctggcccag ctggtcagcg
     4681 acgggagccc catggtccgg aaggagctgg tggtggctct gagtcatctt gtggttcagt
     4741 atgaaagcaa tttctgcacc gtggccctgc agttcataga agaggaaaag aactacgcct
     4801 tgccttctcc agcaaccaca gagggaggga gtttgacccc agtgcgagac agcccgtgca
     4861 cccccagact tcgttctgtg agctcctatg gaaacatccg tgctgtcgcc acagccagga
     4921 gcctcaacaa atctttgcag aacctgagtt tgacagagga atctggtggc gcggtggcgt
     4981 tctcccccgg aaacctcagc accagcagca gcgccagcag caccctgggc agccccgaga
     5041 atgaggagca tatcctgtcc ttcgagacca tcgacaagat gcgccgcgcc agctcctact
     5101 cctccctcaa ctccctcatc ggagtttcct ttaacagtgt ttacactcag atttggagag
     5161 tcctgctgca cctggctgct gacccctatc cagaggtctc ggacgtggcc atgaaagtac
     5221 tcaacagcat cgcctacaag gccaccgtga acgcccggcc gcagcgcgtc ctggacacct
     5281 cctccctcac gcagtcggcc cccgccagcc ccaccaacaa gggcgtgcac atccaccagg
     5341 cggggggctc ccctccggcg tccagcacca gcagctccag cctgaccaac gatgtggcca
     5401 agcagccggt cagccgagac ttgccttctg gccggccggg caccacaggc cccgctgggg
     5461 cgcagtacac ccctcactcc caccagttcc cccggacacg gaagatgttc gacaagggcc
     5521 cagagcagac tgcggacgac gcggacgatg ctgctggaca caaaagtttc atctccgcca
     5581 cggtgcagac ggggttctgc gactggagcg cccgctattt tgcccagccc gtcatgaaga
     5641 tcccagaaga gcacgacctg gagagtcaga tccgcaagga gcgggagtgg cggttcctgc
     5701 gaaacagccg tgtcaggagg caggcccagc aagtcattca gaagggcatt acgagattgg
     5761 acgaccaaat atttctgaac aggaaccccg gcgtcccctc tgtggtgaaa ttccacccct
     5821 tcacgccgtg catcgctgta gccgacaagg acagcatctg cttttgggac tgggagaaag
     5881 gggagaagct ggattatttc cacaatggga accctcggta cacgagggtc actgccatgg
     5941 agtatctgaa tggccaggac tgctcgcttc tgctgacggc cacagacgat ggtgccatca
     6001 gggtctggaa gaattttgct gatttggaaa agaacccaga gatggtgacc gcgtggcagg
     6061 ggctctcgga catgctgcca acgacgcgag gagctgggat ggtggtggac tgggagcagg
     6121 agaccggcct cctcatgagc tcaggagacg tgcggatcgt ccggatctgg gacacagacc
     6181 gtgagatgaa ggtgcaggac atccctacgg gcgcagacag ctgtgtgacg agtctgtcct
     6241 gtgattccca ccgctcactc atcgtggctg gcctcggtga cggctccatc cgcgtctacg
     6301 acagaaggat ggcactcagc gaatgccgcg tcatgacgta ccgggagcac acagcctggg
     6361 tggtgaaggc ctccctgcag aagcgtcccg acggccacat cgtgagtgtg agcgtcaatg
     6421 gagatgtgcg catctttgat ccccggatgc ctgagtcggt aaatgtgctt cagatcgtga
     6481 aggggctgac ggccctggac atccaccccc aggcggacct gatcgcatgt ggctccgtca
     6541 atcagttcac cgccatctac aacagcagcg gagagctcat caacaacatc aagtactacg
     6601 acggcttcat gggccagcgg gtcggcgcca tcagctgcct ggccttccac ccgcactggc
     6661 ctcacctggc cgtgggaagc aacgactact acatctccgt gtactcggtg gagaagcgtg
     6721 tcagatagga tatcttcgaa ttctcgacct cgagacaaat ggcagtattc atccacaatt
     6781 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     6841 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     6901 tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc
     6961 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     7021 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     7081 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     7141 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     7201 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     7261 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     7321 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     7381 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     7441 gacctctctc cccaggggga tccaccggag cttaccatga ccgagtacaa gcccacggtg
     7501 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     7561 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     7621 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     7681 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     7741 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7801 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7861 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7921 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     7981 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     8041 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     8101 ccgaccgaaa ggagcgcacg accccatgca tcggtacctt taagaccaat gacttacaag
     8161 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     8221 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     8281 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8341 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8401 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8461 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8521 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8581 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8641 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8701 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     8761 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     8821 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     8881 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     8941 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     9001 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     9061 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     9121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     9181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     9241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     9481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    10021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    10081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    10141 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    10201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    10261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    10321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    10381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    10441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    10501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    10741 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    10801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    11281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    11341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
