LOCUS       sequence-148294-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mouse Cln6 with C terminal EGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Thelen M, Damme M, Schweizer M, Hagel C, Wong AM, Cooper JD, Braulke
            T, Galliciotti G
  TITLE     Disruption of the autophagy-lysosome pathway is involved in 
            neuropathology of the nclf mouse model of neuronal ceroid 
            lipofuscinosis.
  JOURNAL   PLoS One. 2012;7(4):e35493. doi: 10.1371/journal.pone.0035493. Epub 
            2012 Apr 20.
  PUBMED    22536393
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(4):e35493. doi"; pages: " 
            10.1371/journal.pone.0035493. Epub 2012 Apr 20"
COMMENT     Sequence Label: pEGFP-Cln6
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(49..252)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(78..98)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        253..556
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccatggtggc gaccggtagc gctagcggat ctgacggttc actaaaccag ctctgcttat
       61 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      121 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
      181 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
      241 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
      301 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
      361 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
      421 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
      481 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
      541 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
      601 ttactattaa taactaatgc atggcggtaa tacggttatc cacagaatca ggggataacg
      661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      781 gtcagaggtg gcgaa
//
