LOCUS       sequence-148528-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F 
            mutation) fused to N-terminal fragment of Cre DNA recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL
  TITLE     Optimized second-generation CRY2-CIB dimerizers and photoactivatable
            Cre recombinase.
  JOURNAL   Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.
  PUBMED    27065233
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063."
COMMENT     Sequence Label: CRY2(L348F)-CreN
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(833..849)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(834..850)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
       61 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      121 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      181 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtgtggcggc
      241 tagtactccg gtattgcggt acccttgtac gcctgtttta tactcccttc ccgtaactta
      301 gacgcacaaa accaagttca atagaagggg gtacaaacca gtaccaccac gaacaagcac
      361 ttctgtttcc ccggtgatgt cgtatagact gcttgcgtgg ttgaaagcga cggatccgtt
      421 atccgcttat gtacttcgag aagcccagta ccacctcgga atcttcgatg cgttgcgctc
      481 agcactcaac cccagagtgt agcttaggct gatgagtctg gacatccctc accggtgacg
      541 gtggtccagg ctgcgttggc ggcctaccta tggctaacgc catgggacgc tagttgtgaa
      601 caaggtgtga agagcctatt gagctacata agaatcctcc ggcccctgaa tgcggctaat
      661 cccaacctcg gagcaggtgg tcacaaacca gtgattggcc tgtcgtaacg cgcaagtccg
      721 tggcggaacc gactactttg ggtgtccgtg tttcctttta ttttattgtg gctgcttatg
      781 gtgacaatca cagattgtta tcataaagcg aattggatag gatcaagctt atcgataccg
      841 tcgacctcga gctcgc
//
