LOCUS       sequence-148543-        9369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Dam fused MBD3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Shimbo T, Du Y, Grimm SA, Dhasarathy A, Mav D, Shah RR, Shi H, Wade 
            PA
  TITLE     MBD3 localizes at promoters, gene bodies and enhancers of active 
            genes.
  JOURNAL   PLoS Genet. 2013 Dec;9(12):e1004028. doi: 
            10.1371/journal.pgen.1004028. Epub 2013 Dec 26.
  PUBMED    24385926
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2013-12"; volume: "9(12):e1004028. doi"; pages: " 
            10.1371/journal.pgen.1004028. Epub 2013 Dec 26"
COMMENT     Sequence Label: pLgw-MBD3-V5-Dam
FEATURES             Location/Qualifiers
     source          1..9369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(91..96,97..114,115..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     153..169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    218..339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(229..248)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     283..302
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(469..843)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(862..909)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(957..1286)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      971..1106
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1025..1044)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1271..1291
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1300..1728)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1410..1431)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1622..1641
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1774..1998
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1987..2004
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2030..2210
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             complement(3575..3616)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3627..3651
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4465..4489)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4542..4780)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(4574..4595)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             complement(5242..5286)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5471..5704
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6197..6322
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6369..6549
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6567..6765)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6592..6612)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6767..7146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7318..7337
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7412..7516
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7517..7585,7586..8377)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7735..7754)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8548..9136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9037..9056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9290..9307
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      181 caagctctag ctagaggtcg acggtataca gacatgataa gatacattga tgagtttgga
      241 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
      301 gctttatttg taaccattat aagctgcaat aaacaagttg gggtgggcga agaactccag
      361 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
      421 caacctttca tagaaggcgg cggtggaatc gaaatctcgt agcacgtgtc agtcctgctc
      481 ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact cccgccccca
      541 cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt tcgtggacac
      601 gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc aggccagggt
      661 gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt cgtcccggac
      721 cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt cggtccagaa
      781 ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc
      841 catggtttag ttcctcacct tgtcgtatta tactatgccg atatactatg ccgatgatta
      901 attgtcaaca cgtgctgatc agatccgaaa atggatatac aagctcccgg gagctttttg
      961 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggcagag
     1021 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     1081 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     1141 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     1201 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     1261 gactttccac accctaactg acacacattc cacagaatta attcgcgtta aatttttgtt
     1321 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     1381 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     1441 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     1501 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     1561 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     1621 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     1681 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc
     1741 tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat
     1801 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
     1861 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     1921 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     1981 ggcaactaga aggcacagtc gaggctgatc agcgggttta aacgggccct gctagagatt
     2041 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     2101 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     2161 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacagtcc
     2221 ggatgcagct ctcgggccat gtgatgaaat gctaggcggc tgtcaaacct ccactctaat
     2281 acttctctct ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gcgggtgttc
     2341 tctccttcat tggcttcttc taccttctct tgctcaactg gtactagctt gtagcaccat
     2401 ccaaaggtca gtggatatct gatccctggc cctggtgtgt agttctgcca atcagggaag
     2461 tagccttgtg tgtggtagat ccacagatca aggatatctt gtcttcgttg ggagtgaatt
     2521 agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa
     2581 gtcattggtc ttaaaggtac ctgaggtgtg actggaaaac ccacctcctc ctcctcttgt
     2641 gcttctagcc aggcacaatc agcattggta gctgctgtat tgctacttgt gattgctcca
     2701 tgtttttcta ggtctcgagt tattttttcg cgggtgaaac gactcctggt ttgtacaaag
     2761 ccagcagttc gtccaccttt ttacgtgtgc cgccgttgct gcttatactg cgtcgaactt
     2821 tgacgacatg caattttgcg cgctgatacc actcacgcgt taacatcgta tcgtgattgg
     2881 agatcagcac tggaatatgg cgctcaacca gaccttcggc gatctccgcc agatgcgctt
     2941 gttgttcaag cgtaaaactg tttgtgtgat acgccgtaaa gttggcggtc gcagacagcg
     3001 gtgcataagg cggatcgcaa tagacgacgg atgcatcatc tgcgcgcgcc atgctatcgg
     3061 cgtaagactc acaatagaaa aaggcattct gcgctttttc agcgaagtga tacaactctg
     3121 cttccgggaa atagggtttt ttgtagcggc cgaacggcac gttaaactca ccgcgcagat
     3181 tgtaacgaca caggccgttg taaccgtagc ggttcaaata taaaaacagt accgcccgac
     3241 ggaacggatc ctggcttttg ttgaactctt cgcggaactg atagtaaacc tcggcgcaat
     3301 ttgtttcggg aacaaacagc tcgcgtgcgg cctgtacgta ctcatcagta cgcatcttca
     3361 caatgttata gagactgatc aggtcgctat tgatatcggc aaggatataa cgagaaaagt
     3421 cggtgttgag aaacaccgac ccggcaccta caaaaggctc aaccagacat tcgcccttgg
     3481 gcaaatgccg tttaatatca tcaagcaggg gatacttgcc ccctgcccac ttcaaaaaag
     3541 cgcgattttt cttcatccgc ggttcgaaat cgatcgtaga atcgagaccg aggagagggt
     3601 tagggatagg cttaccaccg gatcgaacca ctttgtacaa gaaagctggg tcggcgcgcc
     3661 cacccttgac gtgctccatc tccgggtccg ggtcgggctc ctcctcctcc tcctcctcgt
     3721 cttcctcgtc gtcgtcctca gcgcaggcct tgtccagcgg cgcctccccg tcacgggcca
     3781 gctcctccac gtgcgccagc atgtcggcca tcagcgcctc ctccagccgc ttccgcacct
     3841 gctgcaccag ctcttcctgc ttcctgatgt cctcgtcggt caccatgaag gctttgcaca
     3901 ggggctgcgt ggtgttgagc catacgccgg ggttcttctc cacggcggcc gagagctgtc
     3961 ccgtgatggg catggtgcta gtgtgcaggg cgctggcgat ggccgacagc agcgtctcat
     4021 ccgtgcagcc aggtcccacc ccctgcaggc ccttggggag gtccatggtc ttgaccagct
     4081 cctcagcaat gtcgaaggcg ttcaggccgc tcagcttctt ctcccagaag agctggcgcg
     4141 gctggtccac cgccttctgc gggtcgctct tgaccttgtt gctggggtgg ttggtaatct
     4201 tggtcaccgg ctgcttgaag atggacgccg tctggcgcac gggcagcgcc gtgttcaggt
     4261 cgggcttgcc cttgacctgg ttggaggagt cgtagcgcac gcgctggcgg ctcttgttca
     4321 tcttgctcat cagcatcttg cccgtgcgga agtcgaaggt gctcaggtcc atggagccgc
     4381 ccaggtagcg cgccagctgc ggcttgctgc ggaacttctt cccgctcggg ctcttccgct
     4441 ccatggtgaa gggggcggcc gcggagcctg cttttttgta caaacttgtt tgatctagct
     4501 gtgtgtgagt tcttctttct cggtaacttg ttgaaagtat tcagagttct cttcttgtat
     4561 tcaataatta cttcttggca gatttcagta gttgcagttg atttacttgg ttgctggtta
     4621 cttttaattg attcacttta acttgcactt tactgcagat tgtttagctt gttcagctgc
     4681 gcttgtttat ttgcttagct ttcgcttagc gacgtgttca ctttgcttgt ttgaattgaa
     4741 ttgtcgctcc gtagacgaag cgcctctatt tatactccgg cggtcgaggg tactcccggg
     4801 tactgagctt tcagcaagag aacaatgcac ttgtccatcg agctttcagc aagagaacaa
     4861 tgcacttgtc catcgagctt tcagcaagag aacaatgcac ttgtccatcg agctaacgag
     4921 aacaatgcac ttagcggtat cgagaacaat gcacttaata tgcggccgag atctgaattc
     4981 ctgcagcccg ggggatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc
     5041 ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa
     5101 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     5161 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     5221 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     5281 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     5341 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     5401 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     5461 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     5521 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     5581 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     5641 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     5701 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     5761 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     5821 cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac
     5881 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     5941 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     6001 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     6061 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     6121 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     6181 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     6241 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     6301 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     6361 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     6421 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     6481 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     6541 gagagaccca gtacaggcaa aacgcgctgc ttatatagac ctcccaccgt acacgcctac
     6601 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     6661 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     6721 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     6781 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     6841 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     6901 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     6961 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     7021 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     7081 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
     7141 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
     7201 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
     7261 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
     7321 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
     7381 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7501 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7561 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7621 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7681 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7741 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7801 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7861 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7921 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7981 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8041 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8101 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8161 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8221 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8281 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8341 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8401 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9301 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9361 tcattaatg
//
