LOCUS       sequence-148566-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac transposon encoding Neomycin resistance with inverted 
            terminal repeat mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Henssen AG, Henaff E, Jiang E, Eisenberg AR, Carson JR, Villasante 
            CM, Ray M, Still E, Burns M, Gandara J, Feschotte C, Mason CE, 
            Kentsis A
  TITLE     Genomic DNA transposition induced by human PGBD5.
  JOURNAL   Elife. 2015 Sep 25;4. pii: e10565. doi: 10.7554/eLife.10565.
  PUBMED    26406119
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-09-25"; volume: "4. pii: e10565. doi"; pages: " 
            10.7554/eLife.10565"
COMMENT     Sequence Label: PB-EF1-IRES-NEO mutant ITR
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(31..39,40..42,43..617)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(204..223)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     433..450
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tccatcttgt tcaatcattc cggacgccat ggttgtggcc atattatcat cgtgtttttc
       61 aaaggaaaac cacgtccccg tggttcgggg ggcctagacg tttttttaac ctcgactaaa
      121 cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc catacaatgg ggtaccttct
      181 gggcatcctt cagccccttg ttgaatacgc ttgaggagag ccatttgact ctttccacaa
      241 ctatccaact cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta tcttatacac
      301 gtggcttttg gccgcagagg cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg
      361 tcgctacaga cgttgtttgt cttcaagaag cttccagagg aactgcttcc ttcacgacat
      421 tcaacagacc ttgcattcct ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga
      481 agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg gtggaaaata
      541 acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta acgttagggg
      601 ggggggaggg agaggggcgc ggccgcggat ccgatttaaa ttcgaattcg ctagctctag
      661 acatatgtag gcgccggtca cagcttggat ctgtaacggc gcagaacaga aaacgaaaca
      721 aagacgtaga gttgagcaag cagg
//
