LOCUS       sequence-148660-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry and FlpO for monosynaptic circuit mapping and 
            genetic manipulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             241..297
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
       61 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      121 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      181 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggaagcgga
      241 gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg
      301 agccagttcg acatcctgtg caagaccccc cccaaggtgc tggtgcggca gttcgtggag
      361 agattcgaga ggcccagcgg cgagaagatc gccagctgtg ccgccgagct gacctacctg
      421 tgctggatga tcacccacaa cggcaccgcc atcaagaggg ccaccttcat gagctacaac
      481 accatcatca gcaacagcct gagcttcgac atcgtgaaca agagcctgca gttcaagtac
      541 aagacccaga aggccaccat cctggaggcc agcctgaaga agctgatccc cgcctgggag
      601 ttcaccatca tcccttacaa cggccagaag caccagagcg acatcaccga catcgtgtcc
      661 agcctgcagc tgcagttcga gagcagcgag gaggccgaca agggcaacag ccacagcaag
      721 aagatgctga aggccctgct gtccgagggc gagagcatct g
//
