LOCUS       sequence-148701-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express tdTomato under EF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     plasmid for first generation adenovirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAdx-EF1-tdTomato
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(82..105)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(280..299)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     549..567
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(753..773)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cggtgctgcc gatgccatgc cccaggaaca ggtggtggcg gccctcggag cgctcgtact
       61 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggtg tccacgtagt
      121 agtagccggg cagttgcacg ggcttcttgg ccatgtagat ggtcttgaac tccaccaggt
      181 agtggccgcc gtccttcagc ttcagggcct ggtggatctc gcccttcagc acgccgtcgc
      241 gggggtacag gcgctcggtg gaggcctccc agcccatggt cttcttctgc attacggggc
      301 cgtcgggggg gaagttggtg ccgcgcatct tcaccttgta gatcagcgtg ccgtcctgca
      361 gggaggagtc ctgggtcacg gtcaccagac cgccgtcctc gaagttcatc acgcgctccc
      421 acttgaagcc ctcggggaag gacagcttct tgtaatcggg gatgtcggcg gggtgcttca
      481 cgtacgcctt ggagccgtac atgaactggg gggacaggat gtcccaggcg aagggcaggg
      541 ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc
      601 cctcgccctc gatctcgaac tcgtggccgt tcatggagcc ctccatgcgc accttgaagc
      661 gcatgaactc tttgatgacc tcctcgccct tgctcaccat tggcccggga atccaattcc
      721 tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa
      781 ccaagatcca aactcaaaaa gggcaaaatt
//
