LOCUS       sequence-148728-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCS4+-NLS-EGFP-E2A-mCherry-CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Kim JH, Lee SR, Li LH, Park HJ, Park JH, Lee KY, Kim MK, Shin BA, 
            Choi SY
  TITLE     High Cleavage Efficiency of a 2A Peptide Derived from Porcine 
            Teschovirus-1 in Human Cell Lines, Zebrafish and Mice.
  JOURNAL   PLoS One. 2011  . 6(4):e18556.
  PUBMED    21602908
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(4):e18556."
COMMENT     Sequence Label: pSYC-103
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..99
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             109..816
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(254..272)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     522..541
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     716..739
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gagctgtaca agcttaagga gctcgcatgc ggaagcggac agtgtactaa ttatgctctc
       61 ttgaaattgg ctggagatgt tgagagcaac cctggaccta gatctaccat ggtgagcaag
      121 ggcgaggagg acaacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      181 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      241 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      301 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      361 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      421 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
      481 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      541 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      601 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc
      661 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagctg
      721 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc
      781 cgccactcca ccggcggcat ggacgagctg tacaagaagc tgaaccctcc tgatgagagt
      841 ggccccggct gcatgagctg caagtgtgtg ctctcctgag ct
//
