LOCUS       sequence-148804-       10128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation Self-inactivating lentivector expressing NS4A from 
            Zika virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10128)
  TITLE     Arumugaswami Lab Plasmids
REFERENCE   2  (bases 1 to 10128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV_Zika_Flag_NS4A
FEATURES             Location/Qualifiers
     source          1..10128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             348..528
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    575..700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1193..1426
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1611..1655
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1953..2070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2148..2359
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2372..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2679..2702
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3138..3710
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3305..3322)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3532..3551
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3713..4315
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4212..4232
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4331..4919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4384..4404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4802..4813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5446..5626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5652..5669)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5658..5882
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5928..6356
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6015..6034)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6225..6246
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6365..6385)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6370..6699
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6550..6685
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6612..6631
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6747..6794
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6813..7187
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7317..7438
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7354..7373)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7408..7427
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7487..7503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7487..7503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7500..7522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7511..7527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7535..7541,7542..7559,7560..7565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7580..7601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7718..7735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7889..8477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7969..7988)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8648..9439,9440..9508))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9271..9290
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9509..9613)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9688..9707)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9879..10128,1..130)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      361 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      421 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      481 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      541 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      601 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      661 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      721 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      781 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      841 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      901 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      961 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1021 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1081 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1141 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1201 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1261 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1321 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1381 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1441 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1501 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1561 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1621 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1681 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1741 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1801 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1861 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1921 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1981 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2041 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2101 gtttggttaa ttctagtgga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     2161 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     2221 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2281 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2341 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     2401 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     2461 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     2521 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     2581 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     2641 caagctgtga ccggcgccta ccaccggtca ccatggccga ctacaaagac gacgatgaca
     2701 agtccggggg agcggctttt ggagtgatgg aagccctggg aacactgcca ggacacatga
     2761 cagagagatt ccaggaagcc attgacaacc tcgctgtgct catgcgggca gagactggaa
     2821 gcaggcctta caaagccgcg gcggcccaat tgccggagac cctagagacc ataatgcttt
     2881 tggggttgct gggaacagtc tcgctgggaa tcttcttcgt cttgatgagg aacaagggca
     2941 tagggaagat gggctttgga atggtgactc ttggggccag cgcatggctc atgtggctct
     3001 cggaaattga gccagccaga attgcatgtg tcctcattgt tgtgttccta ttgctggtgg
     3061 tgctcatacc tgagccagaa aagcaaagat agcactcgag gatatcgacg cgtgaagctt
     3121 agcggccgca aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     3181 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     3241 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     3301 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     3361 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     3421 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     3481 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     3541 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     3601 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     3661 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata ccatggccac
     3721 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     3781 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     3841 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3901 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     3961 cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg gccgagttga gcggttcccg
     4021 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4081 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4141 cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg gtgcccgcct tcctggagac
     4201 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4261 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagttcg
     4321 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4381 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4441 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4501 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4561 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4621 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4681 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4741 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4801 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4861 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4921 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     4981 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     5041 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5101 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5161 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     5221 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     5281 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     5341 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     5401 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     5461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     5581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     5641 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5701 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5761 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5821 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5881 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     5941 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6001 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6061 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6121 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6181 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6241 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6301 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     6361 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     6421 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     6481 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     6541 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6601 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     6661 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     6721 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     6781 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     6841 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     6901 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     6961 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     7021 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     7081 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     7141 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     7201 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     7261 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     7321 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7381 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7441 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7501 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7561 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7621 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7681 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7741 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7801 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7861 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7921 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8941 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     9661 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     9721 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     9781 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     9841 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     9901 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     9961 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
    10021 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
    10081 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatca
//
