LOCUS       sequence-148834-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2875 is a vector for multiple integrations at sites sharing 
            homology with Ty3Cons. The selective marker is Kl.URA3. Additionally
            it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2875
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..89
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    559..592
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     853..875
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     868..884
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     894..913
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        894..912
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(956..989)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gccaggcatc aaactaagca gaaggcccct gacggatggc ctttttgcgt ttctacaaac
       61 tctttctgtg ttgtaaaacg acggccagtc ttaagctcgg gccccctggg cggttctgat
      121 aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt ttttttatgg
      181 ggggagttta gggaaagagc atttgtcaga atatttaagg gcgcctgtca ctttgcttga
      241 tatatgagaa ttatttaacc ttataaatga gaaaaaagca acgcacttta aataagatac
      301 gttgcttttt cgattgatga acacctataa ttaaactatt catctattat ttatgatttt
      361 ttgtatatac aatatttcta gtttgttaaa gagaattaag aaaataaatc tcgaaaataa
      421 taaagggaaa atcagttttt gatatcaaaa ttatacatgt caacgataat acaaaatata
      481 atacaaacta taagatgtta tcagtattta ttatgcattt agaataaatt ttgtgtcgcc
      541 cttattcgac tcactataga agttcctatt ctctagaaag tataggaact tcacttcagg
      601 tctgagtgcg gccgcagatc ttgttgtatt acgggctcga gtaataccgg agtgtcttga
      661 caatcctaat ataaacagtc ttagggaagt aaccagttgt caaaacagtt tatcagatta
      721 attcacggaa tgttacttat cttatatatt atataaaata tgaatcatac taagtggtgg
      781 aagcgcggaa tctgatcaat taatgcctca gcactaatga cgtctgtcct gcaggggtaa
      841 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
      901 actcactata gggcgaattg ggtgcatagg ccactagtgg atctgatatc acctaataac
      961 ttcgtatagc atacattata cgaagttata ttaagggttc tcgagagctc gttttattta
     1021 ggttctatcg agga
//
