LOCUS       sequence-148902-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for AsCpf1 guide RNA (need to clone in 
            spacer into BsmBI sites).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, 
            McCaw ZR, Aryee MJ, Joung JK
  TITLE     Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human 
            cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.
  PUBMED    27347757
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620."
COMMENT     Sequence Label: BPK3079
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(195..443)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(253..272)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(423..443)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        550..654
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(873..892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt catatgtaga
      121 gaggtacctc gagcggccca agcttaaaaa aaggagacgc attaatcgtc tccatctaca
      181 agagtagaaa ttacggtgtt tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa
      241 tactttcaag ttacggtaag catatgatag tccattttaa aacataattt taaaactgca
      301 aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt
      361 acagtcaaat taattctaat tatctctcta acagccttgt atcgtatatg caaatatgaa
      421 ggaatcatgg gaaataggcc ctcttcctgc ccgaccttgg taccggatcc agtcgactga
      481 attggttcct ttaaagcctg cttttttgta cagctagcga cgtcaggtgg cacttttcgg
      541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      901 gacgccgggc a
//
