LOCUS       sequence-148977-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2800 is a vector for multiple integrations at sites sharing 
            homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally
            it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2800
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..799
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHE
                     YVNAAGITHGMDELYK"
     primer_bind     complement(116..144)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 attacaactt tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt
       61 ttaattacaa atctgtcata aaacaatgag caaaggagaa gaacttttca ctggagttgt
      121 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagaggaga
      181 gggtgaaggt gatgctacaa tcggaaaact cacccttaaa tttatttgca ctactggaaa
      241 actacctgtt ccatggccaa cacttgtcac tactctgacc tatggtgttc aatgcttttc
      301 ccgttatccg gatcacatga aaaggcatga ctttttcaag agtgccatgc ccgaaggtta
      361 tgtacaggaa cgcactatat ctttcaaaga tgacgggaaa tacaagacgc gtgctgtagt
      421 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtactg attttaaaga
      481 agatggaaac attctcggac acaaactcga gtacaacttt aactcacaca atgtatacat
      541 cacggcagac aaacaaaaga atggaatcaa agctaacttc acagttcgcc acaacgttga
      601 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      661 tgtcctttta ccagacaacc attacctgtc gacacaaact gtcctttcga aagatcccaa
      721 cgaaaagcgt gaccacatgg tccttcatga gtacgtaaat gctgctggga ttacacatgg
      781 catggatgag ctctacaaat gaatcgcgtg cattcatccg ctctaaccga aaaggaagga
      841 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
      901 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac
      961 attatactga aaaccttgct tgagaaggtt t
//
