LOCUS       sequence-149001-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A 
            mutations. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B,
            Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
  TITLE     In vivo genome editing using Staphylococcus aureus Cas9.
  JOURNAL   Nature. 2015 Apr 1. doi: 10.1038/nature14299.
  PUBMED    25830891
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Apr 1. doi: 10.1038/nature14299."
COMMENT     Sequence Label: 
            pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..90)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(97..144)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gaattcttag gcatagtcgg ggacatcata tgggtatgca taatcaggca cgtcgtaggg
       61 ataagcgtaa tctggaacat cgtatgggta ggatcccttt ttcttttttg cctggccggc
      121 ctttttcgtg gccgccggcc ttttgccctt tttgatgatc tgagggtgct tcttagattt
      181 cacttcatac aggttgccca gaatgtctgt gctgtacttc ttaatgctct gggtcttgga
      241 ggcgattgtc ttaatgatcc tggggggcct cttgtcgttc atgttttcca ggtactcgcg
      301 gtaggtgatg tcgatcatgt tcacttcgat ccggttcagc aggtcgttgt tcacgccgat
      361 cactctatac agctcgccgt tgatcttgat cagatcgttg ttgtagaagg aggcgataaa
      421 ctcggcctgg ttgctgatct tcttcagctt cttagcttcc tcatagcact tgctattcac
      481 ttcgtagtag ttttcttttt tgatcacatc cagattcttc acggtcacga acttgtacac
      541 gccattgtcc aggtacacgt cgaatctgta gggcttcagg gacagcttca cgaccttgtt
      601 tctgctgttg gggtagtcgt cggtgatgtc cagatgggcg ttcagtttgt tgccgtaata
      661 cttaatcttc ttgatcacgg ggccgttgtc ctttttggag tacttggtca ggtagttccc
      721 ggtttcctcg tagtacttgt acaggggatt cttctcgtcg ccgtactgtt ccataatcag
      781 cttcagtttc tggtaggtct gggggtcgtg gtggtacatc agcagctttt cggggctctt
      841 gttgatcagc ttttt
//
