LOCUS       sequence-149010-        1137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MK03. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MK03_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcggcgg cggcggctca ggggggcggg ggcggggagc cccgtagaac cgagggggtc
       61 ggcccggggg tcccggggga ggtggagatg gtgaaggggc agccgttcga cgtgggcccg
      121 cgctacacgc agttgcagta catcggcgag ggcgcgtacg gcatggtcag ctcggcctat
      181 gaccacgtgc gcaagactcg cgtggccatc aagaagatca gccccttcga acatcagacc
      241 tactgccagc gcacgctccg ggagatccag atcctgctgc gcttccgcca tgagaatgtc
      301 atcggcatcc gagacattct gcgggcgtcc accctggaag ccatgagaga tgtctacatt
      361 gtgcaggacc tgatggagac tgacctgtac aagttgctga aaagccagca gctgagcaat
      421 gaccatatct gctacttcct ctaccagatc ctgcggggcc tcaagtacat ccactccgcc
      481 aacgtgctcc accgagatct aaagccctcc aacctgctca tcaacaccac ctgcgacctt
      541 aagatttgtg atttcggcct ggcccggatt gccgatcctg agcatgacca caccggcttc
      601 ctgacggagt atgtggctac gcgctggtac cgggccccag agatcatgct gaactccaag
      661 ggctatacca agtccatcga catctggtct gtgggctgca ttctggctga gatgctctct
      721 aaccggccca tcttccctgg caagcactac ctggatcagc tcaaccacat tctgggcatc
      781 ctgggctccc catcccagga ggacctgaat tgtatcatca acatgaaggc ccgaaactac
      841 ctacagtctc tgccctccaa gaccaaggtg gcttgggcca agcttttccc caagtcagac
      901 tccaaagccc ttgacctgct ggaccggatg ttaaccttta accccaataa acggatcaca
      961 gtggaggaag cgctggctca cccctacctg gagcagtact atgacccgac ggatgagcca
     1021 gtggccgagg agcccttcac cttcgccatg gagctggatg acctacctaa ggagcggctg
     1081 aaggagctca tcttccagga gacagcacgc ttccagcccg gagtgctgga ggccccc
//
