LOCUS       sequence-149107-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Nguyen PT, Li QW, Kadaba NS, Lai JY, Yang JG, Rees DC
  TITLE     The contribution of methionine to the stability of the Escherichia 
            coli MetNIQ ABC transporter-substrate binding protein complex.
  JOURNAL   Biol Chem. 2015 Sep;396(9-10):1127-34. doi: 10.1515/hsz-2015-0131.
  PUBMED    25803078
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Chem."; date: "2015-09"; volume: "396(9-10):1127-34. doi"; pages: " 
            10.1515/hsz-2015-0131"
COMMENT     Sequence Label: Lf-MetQ
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(18..446)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(128..149)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     340..359
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(483..530)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     526..544
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(597..614)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
       61 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      121 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      181 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      241 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
      301 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
      361 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
      421 gcttaatgcg ccgctacagg gcgcgtccca ttcgccaatc cggatatagt tcctcctttc
      481 agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca
      541 gcggtggcag cagccaactc agcttccttt cgggctttgt tagcagccgg atctcagtgg
      601 tggtggtggt ggtgctcgag ccagccttta acagctccgc cgttaaacac tttgtttgct
      661 gcttcgtaaa cttcgtcaga ctgataagcc tggacgaatt tcttcacgtt ctcggcgtct
      721 ttgttatctt cacgcgtcac gatcaggttt acgtacgggg actctttatc ttcaacaaag
      781 ataccgtctt tcgccggagt caggccaatc tggctggcat aggtggtatt gataactgcc
      841 agagcgattt gcgcgtcgtc cagagaacgc ggca
//
