LOCUS       sequence-149247-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Denisov IG, Grinkova YV, Lazarides AA, Sligar SG
  TITLE     Directed self-assembly of monodisperse phospholipid bilayer 
            Nanodiscs with controlled size.
  JOURNAL   J Am Chem Soc. 2004 Mar 24. 126(11):3477-87.
  PUBMED    15025475
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2004 Mar 24. 126(11):3477-87."
COMMENT     Sequence Label: pMSP1
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(668..679)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(680..697)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             710..732
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    747..771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(771..790)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(772..790)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(875..894)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttattactg ggtattcagc tttttagtat attcttccag agctgacaga aaactgactt
      121 taaagctctc aagaacaggc aataagccct ggcgtagatc ttccagcgcc ggtttcgctt
      181 tttcgctcaa ggtgctcagg tgttctgtcg ctttcgcgtg gtactcagcc aagcgggcac
      241 ccccgttttc tttcagcgcc tcgagacgtg cggccaaacg ctggcgaagt tcatccgaat
      301 acggcgccaa atgagtccgg agtgcatcaa catgtgcacg ggcgcgatcg cgcatttctt
      361 cgcctaatgg gctgagcttc tcttggagct catggagttt ttggcgtgcc ccctcttgca
      421 gttccgcacg cagcggttcc accttttgac gatataattc catctcttcc tgccattttt
      481 tctgaaagtc atctagatat ggctgaacct tggccttcac ctcttctaaa tctttggaca
      541 tttcctgacg cagtccctcg gtttcttttt ccaggttgtc ccagaattcc tgcgtcacgg
      601 ggcccagttg ttcgcgaagt ttactgaagg tagacgtaac agagtcccaa ttgtccaaca
      661 gcttcagacg tccctcaatg tgatgatgat gatgatgacc catggtatat ctccttctta
      721 aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg
      781 agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg
      841 gcatcaccgg cgcccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
      901 gaaaatcggg ctcgccactt
//
